期刊
CRITICAL REVIEWS IN MICROBIOLOGY
卷 31, 期 4, 页码 191-196出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/10408410500304041
关键词
PCR-RFLP; molecular typing; Brucella; Brucellosis; omp2; omp25; omp31; dnaK; ery; rpsL
类别
Brucellosis is a worldwide zoonosis causing reproductive failures in livestock and a severe multi-organ disease in humans. The genus Brucella is divided into seven species and various biotypes differing in pathogenicity and host specificity. Although Brucella spp. represent a highly homogenous group of bacteria, RFLPs of selected genes display sufficient polymorphism to distinguish Brucella species and biovars. PCR-RFLP analysis shows excellent typeability, reproducibility, stability, and epidemiological concordance. Consequently, PCR-RFLP assays of specific gene loci can serve as tools for diagnostic, epidemiological, taxonomic, and evolutionary studies. Various PCR-RFLPs used for the identification of Brucella species and biotypes are reviewed.
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