期刊
CARBOHYDRATE POLYMERS
卷 112, 期 -, 页码 556-562出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2014.06.035
关键词
Leuconostoc mesenteroides BD1710; Tomato juice sucrose medium; Dextran
资金
- National High Technology Research and Development Program of China (863 Program) [2011AA100901]
The characteristics of the growth of Leuconostoc mesenteroides BD1710 and the synthesis of dextran in tomato juice supplemented with 15% sucrose were assayed. L. mesenteroides BD1710 could synthesize approximately 32 g L-1 dextran in the tomato-juice-sucrose medium when cultured at 28 degrees C for 48 h, which was on the same level as the dextran yield in a chemically defined medium. The viscosity of the cultured tomato-juice-sucrose medium with various dextran contents was also measured. The results of the monosaccharide composition, molecular-weight distribution, Fourier transform infrared spectra (FTIR) and nuclear magnetic resonance spectra (NMR) showed that the polysaccharide synthesized by L. mesenteroides BD1710 in the tomato-juice-sucrose medium was dextran with a peak molecular weight of 635 x 10(5) Da, a linear backbone composed of consecutive alpha-(1 -> 6)-linked o-glucopyranosyl units and approximately 6% alpha-(1 -> 3) branches. (C) 2014 Elsevier Ltd. All rights reserved.
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