期刊
CARBOHYDRATE POLYMERS
卷 111, 期 -, 页码 485-491出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2014.05.013
关键词
Mangrove fungus; Penicillium solitum; Extracellular polysaccharide; NMR; Structure
资金
- Science and Technology Development Program of Shandong Province, China [2010GHY10509]
- National Oceanographic Center of Qingdao of China
- National Natural Science Foundation of China [41076086]
A water soluble extracellular polysaccharide, designated GW-12, was obtained from the liquid culture broth of the mangrove fungus Penicillium solitum by ethanol precipitation, anion-exchange and size-exclusion chromatography. Reversed-phase high performance liquid chromatography analysis showed that GW-12 mainly consisted of D-mannose, and its molecular weight was estimated to be about 11.3 kDa determined by high performance gel permeation chromatography. On the basis of chemical and spectroscopic analyses, including methylation analysis and nuclear magnetic resonance (NMR) spectroscopy, the structure of GW-12 may be represented as a mannan with branches. The main chain of GW-12 was composed of (1 -> 2)-linked alpha-D-mannopyranose and (1 -> 6)-linked alpha-D-mannopyranose residues, branched by single alpha-D-mannopyranose units attached to the main chain at C-6 positions of (1 -> 2)linked alpha-D-mannopyranose residues. There was three branch points for every seven sugar residues in the backbone. (C) 2014 Elsevier Ltd. All rights reserved.
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