期刊
CARBOHYDRATE POLYMERS
卷 113, 期 -, 页码 182-188出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2014.06.089
关键词
Starch; alpha-Amylase; Log of slope plot; First-order kinetics; FTIR-ATR; Retrogradation
资金
- BBSRC (DRINC) [BB/H004866/1]
- BBSRC
- BBSRC [BB/H004866/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [1060665, BB/H004866/1] Funding Source: researchfish
The rate and extent of digestibility of starch were analysed using the logarithm of the slope (LOS) method. Digestibility curves with a-amylase were obtained for starches in their native, gelatinised and 24 h retrograded form. A LOS plot of the digestibility curves was then constructed, which allowed the rate constant (k) and the concentration of the product at the end of the reaction (C infinity) to be calculated. It also allowed the identification of rapid and slow phases in starch digestion. Upon gelatinisation, both k and C infinity increased with dramatic changes notably in C infinity; however after starch samples had been stored for 24 h at room temperature, k was not affected but C infinity decreased. This suggests that retrograded starch is virtually inert to amylase action. Both k and Coo were strongly related to the increase in degree of order of the alpha-glucan chains, monitored by FTIR-ATR spectroscopy, in retrograded starch. (C) 2014 The Authors. Published by Elsevier Ltd.
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