期刊
CARBOHYDRATE POLYMERS
卷 102, 期 -, 页码 901-911出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2013.10.060
关键词
Chitosan; Collagen; Carbon dioxide; Porous scaffold; Genipin
资金
- Foundation for Polish Science
- European Regional Development Fund under the Operational Program Innovative Economy [VENTURES/2010-5/2]
- Polish Ministry of Science [N N405 420739, N N312 258540]
Novel porous scaffolds composed of chitosan, collagen and gelatin were prepared by the multistep procedure involving final freeze-drying and characterized. To eliminate the need for residual acid removal from the material after drying, carbon dioxide saturation process was used for chitosan blend formulation. The use of CO2 for chitosan dissolution made the scaffold preparation process more reproducible and economically sustainable. Genipin was applied to stabilize the structure of the scaffolds and those crosslinked at a level of 7.3% exhibited a homogenous porous structure (33.1%), high swelling capacity (27.6 g/g for wound exudate like medium; 62.5 g/g for water), and were stable under cyclic compression. The values of other investigated parameters: dissolution degree (30%), lysozyme-induced degradation (5% after 168 h), good antioxidant properties (DPPH, ABTS, Fe2+ assays) and especially very low in vitro cytotoxicity against fibroblasts (103%, MTT assay), were highly advantageous for possible biomedical applications of the novel materials. (C) 2013 Elsevier Ltd. All rights reserved.
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