期刊
CARBOHYDRATE POLYMERS
卷 102, 期 -, 页码 649-652出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2013.11.010
关键词
Chitin deacetylase; Rhodococcus erythropolis; Statistical optimization
资金
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- National Natural Science Foundation of China [41306165]
- China Postdoctoral Foundation Funding [2011M501169]
A strain producing chitin deacetylase (CDA) was isolated and identified as Rhodococcus erythropolis by morphological characteristics and 16S rDNA analysis, named as R. erythropolis HG05. By Plackett-Burman and central composite design, CDA production from R. erythropolis HG05 was increased from 58.00 U/mL to 238.89 U/mL. With the crude enzyme from R. erythropolis HG05, the hydrolysate components from colloid chitin were chito-oligosaccharides with polymerization number larger than hexaose. (C) 2013 Elsevier Ltd. All rights reserved.
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