期刊
CARBOHYDRATE POLYMERS
卷 107, 期 -, 页码 221-231出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2014.02.074
关键词
Catalytic synthesis; Sulfated polysaccharides; Solution conformation; Antioxidant activities; Artemisia sphaerocephala
资金
- National Science Foundation of China [21204073, 51273162]
- Natural Science Foundation of Gansu Province [1308RJZA200]
- Promotion Plan of Scientific Research Ability for Young Teachers from Northwest Normal University
Sulfated derivatives of Artemisia sphaerocephala polysaccharide (ASP) with high degree of substitution (DS) were synthesized using 4-dimethylaminopyridine (DMAP)/dimethylcyclohexylcarbodiimide (DCC) as catalyst. Size exclusion chromatography combined with multi-angle laser light scattering (SEC-LLS) results showed a decrease in fractal dimension (d(l)) values of sulfated ASP (SASP). Compared to ASP and SASP with low DS (0.51-1.01), SASP(cata2) exhibited an internal structure between rigid rod and random coil with a DS of 1.24. DS had greater influence on its conformation in aqueous solution. Circular dichroism (CD), methylene blue (MB) and Congo red (CR) spectrophotometric method and atomic force microscopy (AFM) results confirmed that the degradation of ASP and -SO3H groups improved significantly the stiffness of the chains due to the electrostatic effect. Furthermore, antioxidant experiments revealed that high DS could enhance the scavenging activities of radicals and reducing power of SASP in vitro. The extended chain conformation was beneficial to enhance the biological activity of sulfated polysaccharides. Crown Copyright (C) 2014 Published by Elsevier Ltd. All rights reserved.
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