期刊
ANNUAL REVIEW OF ENTOMOLOGY
卷 50, 期 -, 页码 71-100出版社
ANNUAL REVIEWS
DOI: 10.1146/annurev.ento.50.071803.130456
关键词
linkage maps; mutation; sex determination; EST database; genetic engineering
类别
We review progress in applying molecular genetic and genomic technologies to studies in the domesticated silkworm. Bombyx mori, highlighting its use as a model for Lepidoptera, and in sericulture and biotechnology. Dense molecular linkage maps are being integrated with classical linkage maps for positional cloning and marker-assisted selection. Classical mutations have been identified by a candidate gene approach. Cytogenetic and sequence analyses show that the W chromosome is composed largely of nested full-length long terminal repeat retrotransposons. Z-chromosome-linked sequences show a lack of dosage compensation. The downstream sex differentiation mechanism has been studied via the silkworm homolog of double-sex Expressed sequence tagged databases have been used to discover Lepidoptera-specific genes, provide evidence for horizontal gene transfer, and construct microarrays. Physical maps using large-fragment bacterial artificial chromosome libraries have been constructed, and whole-genome shotgun sequencing is underway. Gem-dine transformation and transient expression systems are well established and available for functional studies, high-level protein expression, and gene silencing via RNA interference.
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