Selective adsorption of hemoglobin using polymer-grafted-magnetite nanocellulose composite

A novel adsorbent, poly(methacrylic acid-co-vinyl sulfonic acid)-grafted-magnetite nanocellulose composite (P(MAA-co-VSA)-g-MNCC) was synthesized for adsorbing hemoglobin (Hb) selectively from aqueous solutions. FTIR, XRD and DLS analyses were carried out to characterize the material. Hb exhibited a decrease in alpha-helix and an increase in beta-sheet structure, upon immobilization onto P(MAA-co-VSA)-g-MNCC. The maximum adsorption was found to be at pH 6.5 with a monolayer capacity of 248.19 mg/g at 30 degrees C. Adsorption capacity attained saturation within 2 h. The kinetic data were found to follow pseudo-second-order model which is based on chemisorption. Adsorption behavior was observed to be endothermic in nature. P(MAA-co-VSA)-g-MNCC can be used in the selective adsorption of Hb from mixture of proteins. Spent adsorbent was effectively regenerated with 0.01 M KOH. Present investigation had shown that P(MAA-co-VSA)-g-MNCC would be a promising material for the selective recovery of Hb from aqueous solutions. (c) 2012 Elsevier Ltd. All rights reserved.