Optimization of extraction process of crude polysaccharides from Pomegranate peel by response surface methodology

In this study, response surface methodology was employed to optimize the extraction process of crude polysaccharides from Pomegranate peel with water. Three independent and main variables, including extraction time (h), extraction temperature (degrees C) and ratio of water to raw material (ml/g), which were of significance for the yields of polysaccharides were studied and the Box-Behnken design was based on the results of a single-factors test. The experimental data were fitted to a second-order polynomial equation using multiple regression analysis and also examined using the appropriate statistical methods. The best extraction conditions are as follows: extraction time 1.9 h, extraction temperature 98 degrees C, ratio of water to raw material 37 ml/g. Under the optimization conditions, the experimental yield was 10.358%, which was well matched with the predictive yield of 10.423%. (C) 2012 Elsevier Ltd. All rights reserved.