期刊
CARBOHYDRATE POLYMERS
卷 95, 期 1, 页码 465-470出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2013.02.044
关键词
Continuous enzymatic packed-bed reactor; beta-D-Galactosidase; Chitosan macroparticles; Galactooligosaccharides; Lactose hydrolysis
资金
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
- Fundacao de Amparo a Pesquisa do Rio Grande do Sul (FAPERGS)
beta-D-Galactosidase from Kluyveromyces lactis was immobilized on glutaraldehyde-activated chitosan and used in a packed-bed reactor for the continuous hydrolysis of lactose and the synthesis of galactooligosaccharides (GOS). The biocatalyst was tested for its optima pH and temperature, thermal stability in the presence of substrate and products, and operational stability. Immobilization increased the range of operational pH and temperature, and the enzyme thermal stability was sharply increased in the presence of lactose. Almost complete lactose hydrolysis was achieved for both milk whey and lactose solution at 37 degrees C at flow rates up to 2.6 mL min(-1). Maximal GOS concentration of 26 g L-1 was obtained at a flow rate of 3.1 mL min(-1), with a productivity of 186 g L-1 h(-1). Steady-state operation for 15 days showed the reactor stability concerning lactose hydrolysis. (c) 2013 Elsevier Ltd. All rights reserved.
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