期刊
CARBOHYDRATE POLYMERS
卷 97, 期 2, 页码 523-529出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2013.05.019
关键词
Pulp fiber; Enzyme immobilization; Pectinase; Water treatment; Sodium periodate
资金
- National Natural Science Foundation of China [31270636, 31200457]
- Foundation for the Author of Excellent Doctoral Dissertation of Guangdong Province [Sybzzxm201028]
- SCUT from the college' basic research and operation of MOE [2012ZM0075, 2011ZB0012]
- Program for New Century Excellent Talents in University [NCET-12-0192]
Modified pulp fiber was originally used as a new type of carrier for pectinase immobilization. Pulp fiber was oxidized by sodium periodate to produce aldehyde groups for covalently binding with amino groups of pectinase. Results showed that the enzymatic activity of immobilized pectinase on pulp fiber reached 65 mu g g(-1) min(-1) when immobilization pH value, temperature and time were of 7.0, 20 degrees C and 15 min, respectively. The immobilized pectinase showed higher thermo stability in a wider temperature range of 40-70 degrees C than its free type and its optimal pH shifted from 8.0 to 8.8. Furthermore, the immobilized pectinase exhibited good operational stability. When employed in whitewater treatment of papermaking industry, it still efficiently decreased the cationic demand after operating repeatedly for six batches. The results obtained demonstrate a promising route to prepare available, cheap and biodegradable carrier for immobilizing enzymes with potential application in wastewater treatment in papermaking industry. (C) 2013 Elsevier Ltd. All rights reserved.
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