期刊
CARBOHYDRATE POLYMERS
卷 94, 期 1, 页码 409-415出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2013.01.066
关键词
Bacillus halodurans; Lignocellulosic wastes; Cellulase; Bioconversion; Haloalkaline; Organic solvents
An extracellular thermostable, haloalkaline cellulase by bioconversion of lignocellulosic wastes from Bacillus halodurans CAS 1 was purified to homogeneity with recovery of 12.54% and purity fold 7.96 with the molecular weight of 44 kDa. The optimum temperature, pH and NaCl for enzyme activity was determined as 60 degrees C, 9.0 and 30% and it retained 80% of activity even at 80 degrees C, 12 and 35% respectively. The activity was greatly inhibited by EDTA, indicating that it was a metalloenzyme and significant inhibition by PMSF revealed that serine residue was essential for catalytic activity. The purified cellulase hydrolyzed CMC, cellobiose and xylan, but not avicel, cellulose and PNPG. Furthermore, the cellulase was highly stable in the presence of detergents and organic solvents such as acetone, n-hexane and acetonitrile. Thus, the purified cellulase from B. halodurans utilizing lignocellulosic biomass could be greatly useful to develop industrial processes. Published by Elsevier Ltd.
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