期刊
CARBOHYDRATE POLYMERS
卷 95, 期 1, 页码 282-287出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2013.02.057
关键词
Grifola frondosa; Sulfated polysaccharides; Apoptosis; Notch1; NF-kappa B
资金
- Key Projects in National Science & Technology Pillar Program during the 12th Five-Year Plan Period [2012BAD33B04]
- plan for the development of Changjiang Scholars and Innovative Research Team [IRT1166]
- National Nature Science Foundation of China [31000768]
Sulfated polysaccharides have been known to inhibit proliferation in tumor cells. However, the molecular mechanisms involved in sulfated polysaccharides-induced apoptosis are still uncharacterized. In this study, the effect of a chemically sulfated polysaccharide obtained from Grifola frondosa (S-GFB) on HepG2 cell proliferation and apoptosis-related mechanism were investigated. It was found that S-GFB inhibited proliferation of HepG2 cells in a dose-dependent manner with IC50 at 48 h of 61 mu g ml(-1). The results of scanning electron micrographs indicated that S-GFB induced typical apoptotic morphological feature in HepG2 cells. Flow cytometric analysis demonstrated that S-GFB caused apoptosis of HepG2 cells through cells arrested at S phase. Western-blotting results showed that S-GFB inhibited notch1 expression, I kappa B-alpha degradation and NF-kappa B/p65 translocation from cytoplasm into nucleus. Simultaneously, the apoptotic mechanism of HepG2 cells induced by S-GFB was associated with down regulation of FLIP, and activation of caspase-3 and caspase-8. Taken together, these findings suggest that the S-GFB induces apoptosis through a notch1/NF-kappa B/p65-mediated caspase pathway. Crown Copyright (c) 2013 Published by Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据