期刊
CARBOHYDRATE POLYMERS
卷 97, 期 2, 页码 627-634出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2013.05.039
关键词
Biomass; Endophytic fungi; Exopolysaccharide; Fusarium solani; Optimization; Submerged fermentation
资金
- University Grants Commission (UGC), New Delhi [40-131/2011]
- UGC, New Delhi, India [F-4-3/2006(BSR)/11-114/2008(BSR)]
Endophytic fungi were less investigated for exopolysaccharide production. In this study endophytic Fusarium solani SD5 was used for optimization of exopolysaccharide production. One variable at a time method and response surface methodology were employed to explore the optimum medium compositions and fermentation conditions. The organism produced maximum exopolysaccharide after 13.68 days of incubation at 28 degrees C in potato dextrose broth supplemented with (g%/l) glucose, 9.8; yeast extract, 0.69; KCl, 0.05; KH2PO4, 0.05 with medium pH 6.46. Use of 50 ml medium in 250 ml Erlenmeyer flask gives highest exopolysaccharide production. The organism produced more than two times higher exopolysaccharide (2.276 +/- 0.032 g/l EPS) at optimized condition compared to pre-optimized condition (0.96 +/- 0.021). In vivo toxicity test established nontoxic nature of the EPS (<= 400 mg EPS/Kg of body weight). The EPS slightly altered intestinal indigenous bacteria and influenced the growth of beneficial Lactobacillus spp. (C) 2013 Elsevier Ltd. All rights reserved.
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