期刊
CARBOHYDRATE POLYMERS
卷 92, 期 2, 页码 1991-1996出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2012.11.088
关键词
Enteromorpha prolifera; Sulfated polysaccharide; Microwave-assistance; Molecular weight; Antioxidant activity
资金
- National High Technology Research and Development Program (863 Program) of China [2011AA09070405]
- commonweal item of State Oceanic Administration People's Republic of China [201105028-03]
The effects of degradation on molecular weights (Mws) of polysaccharides from Enteromorpha prolifera were investigated. Microwave-assistance could highly accelerate reaction rate. Six representative sulfated polysaccharides (Mw 446.5, 247.0, 76.1, 19.0, 5.0 and 3.1 KDa) were prepared by a microwave-assistance acid hydrolysis method. Chemical analysis and FT-IR spectrum showed only glycosidic linkages were cleft without breaking significant structural units. Antioxidant activities of representative polysaccharides revealed that all samples showed great inhibitory effects on superoxide radical at a low concentration compared to Vitamin C and samples with high Mws exhibited higher inhibitory effects. On the contrary, samples with low Mws possessed stronger inhibitory effects on hydroxyl radical, IC50 of Mw 3.1 KDa was 0.39 mg/mL. The chelating effect of Mw 3.1 KDa was 77.3% at 5 mg/mL, which was twice more than initial polysaccharide. The study indicated Mw was the most significant factor to influence antioxidant activities of polysaccharides from E. prolifera. (C) 2012 Elsevier Ltd. All rights reserved.
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