期刊
CARBOHYDRATE POLYMERS
卷 92, 期 2, 页码 2239-2244出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2012.11.069
关键词
Pleurotus eryngii; Heteropolysaccharide; Structural elucidation; NMR analysis
资金
- Zhejiang major science and technology projects and special priority themes [2009C13029]
A novel water-soluble heteropolysaccharide (PEPS1), with a molecular weight of 1.88 x 10(4) Da as determined by high performance liquid chromatography (HPLC), specifically size exclusion chromatography, was isolated from the fruiting bodies of Pleurotus eryngii by hot water extraction and further purification by DEAE Sepharose Fast Flow and Sephacryl S-300 and S-100 High-Resolution chromatography. By use of compositional analysis, methylation analysis, together with H-1, C-13 NMR and 2D NMR spectroscopy including COSY, TOCSY, HMQC, HMBC and NOESY experiments, the PEPS1 was identified as a heteropolysaccharide that consists of a alpha-D-(1 -> 6)-Galactopyranan backbone with a beta-D-Mannosyl unit on O-2 of the 2,6-di-O-substituted-D-Galactosyl units, alpha-(1 -> 6)-3-O-Me-D-Galactopyranan backbone with a terminal alpha-3-O-Me-D-Galactosyl unit and it also contained a minor 2,3,6-alpha-D-Galatopyranan units and 4-beta-D-Mannopyranan residues. (C) 2012 Elsevier Ltd. All rights reserved.
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