Optimization of extraction of Eucommia ulmoides polysaccharides by response surface methodology

In this study, extraction yield of Eucommia ulmoides polysaccharides was optimized by the utilization of response surface methodology (RSM). Based on contour plots and variance analysis, optimum operational conditions for maximizing extraction yield were found to be extraction time 80 min, ratio of water to raw material 3, and extraction number 3. Then, we investigated the protective effect of the E. ulmoides polysaccharides on the tissue peroxidative damage and abnormal antioxidant levels in ischemia reperfusion (IR) induced renal toxicity in male albino rabbits. Decrease in all the enzymes (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR)) and non-enzymatic antioxidant (glutathione (GSH)), along with an increase in the lipid peroxidative index (malondialdehyde) was found in all the renal ischemia reperfusion (RIR) rabbits as compared with normal controls. The findings indicate that the extract of E. ulmoides polysaccharides can protect the kidney against IR induced oxidative damage in rabbits. (C) 2012 Elsevier Ltd. All rights reserved.