期刊
CARBOHYDRATE POLYMERS
卷 87, 期 2, 页码 1515-1521出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2011.09.048
关键词
Thermo-sensitive; Alginate; Injectable; Hydrogel; Tissue engineering
资金
- National Natural Science Foundation of China [51072090, 51061130554]
- Doctor Subject Foundation of the Ministry of Education of China [20100002110074]
A thermo-sensitive comb-like copolymer was synthesized by grafting PNIPAAm-COOH with a single carboxy end group onto aminated alginate (AAlg) through amide bond linkages. In the copolymer, alginate was the backbone and poly(N-isopropylacrylamide) (PNIPAAm) was the pendant group. The structures of AAlg and three AAlg-g-PNIPAAm copolymers with different PNIPAAm grafting ratios were determined by FTIR and (1)H NMR. The theological properties of AAlg-g-PNIPAAm copolymer hydrogels were measured by monitoring the viscosity, storage modulus and loss modulus as a function of temperature. The lower critical solution temperature of AAlg-g-PNIPAAm copolymers was measured as 35 C through theological analysis. An in vitro degradation study was carried out by monitoring weight loss. It was confirmed that degradation can be controlled by PNIPAAm modification. Encapsulation of human bone mesenchymal stem cells (hBMSCs) within hydrogels showed that the AAlg-g-PNIPAAm copolymer was not cytotoxic and preserved the viability of the entrapped cells well. The thermo-sensitive AAlg-g-PNIPAAm copolymer has attractive properties that make it suitable as cell or pharmaceutical delivery vehicles for a variety of tissue engineering applications. (C) 2011 Elsevier Ltd. All rights reserved.
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