期刊
OLIGONUCLEOTIDES
卷 15, 期 4, 页码 303-309出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/oli.2005.15.303
关键词
-
The hairpin ribozyme (HPR) is a naturally existing RNA that catalyzes site-specific RNA cleavage and ligation. At 37 degrees C and in the presence of divalent metal ions (M2+), the HPR efficiently cleaves RNA substrates in trans. Here, we show that the HPR can catalyze efficient M2+-independent ligation in trans in aqueous solutions containing any of several alcohols, including methanol, ethanol, and isopropanol, and millimolar concentrations of monovalent cations. Ligation proceeds most efficiently in 60% isopropanol at 37 degrees C, whereas the reverse (cleavage) reaction is negligible under these conditions. We suggest that dehydration of the RNA is the key factor promoting HPR activity in water-alcohol solutions. Alcohol-induced ribozyme ligation may have practical applications.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据