Effects of Astragalus polysaccharide liposome on lymphocyte proliferation in vitro and adjuvanticity in vivo

Two experiments were carried out. In vitro, the effects of Astragalus polysaccharide liposome (APSL) on chicken's T and B lymphocytes proliferation were determined. The results showed that APSL could significantly promote T and B lymphocytes proliferation singly or synergistically with PHA and LPS and the efficacy were superior to those of Astragalus polysaccharide CAPS) and blank liposome (BL) at most concentrations. In immune response experiment, the adjuvanticity of APSL at three doses, APS and BL were compared on chickens vaccinated with ND vaccine. The results showed that APSL could significantly promote lymphocyte proliferation, enhance antibody titer, promote IFN-gamma, IL-2, IL-4 and IL-10 secretion, and its medium dose possessed the best efficacy. These results indicated that APSL could significantly improve the adjuvanticity and drug action of APS, and its medium dose possessed the best efficacy, the liposome would be expected to exploit into a new-type preparation of APS. (C) 2011 Elsevier Ltd. All rights reserved.