Optimisation of exopolysaccharide production by Gomphidius rutilus and its antioxidant activities in vitro

The production conditions of the Gomphidius rutilus exopolysaccharides (GREP) in submerged culture were optimised, and the antioxidant activities of GREP in vitro were evaluated. The optimal culture medium constituents were determined as follows: 30 g/L sucrose, 3.0 g/L soybean meal, 0.25 g/L MgSO4, 1.5 g/L K2HPO4, 0.5 g/L KH2PO4, 0.03 g/L ZnSO4, and 0.01 g/L FeSO4. The optimum parameters for the liquid fermentation were as follows: temperature, 25 degrees C: cultivation time, 6 d; initial pH, 8.0; volume of medium, 150 mL; and rotary speed, 180 rpm. GREP content and dry cell weight in optimised conditions were 540.1 +/- 15.9 mg/L and 8.2 +/- 0.3 g/L, respectively. GREP content under the optimised conditions was 2.5 times than that under the basic culture medium and initial conditions. GREP demonstrated positive antioxidant potential on superoxide anion radical, 1,1-diphenyl-2-picrylhydrazyl, and hydroxyl radical scavenging, and reducing power. (C) 2011 Elsevier Ltd. All rights reserved.