期刊
CARBOHYDRATE POLYMERS
卷 86, 期 3, 页码 1320-1326出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2011.06.036
关键词
Response surface methodology; Polysaccharides; Isatis tinctoria L.; Optimization; Extraction; Free radicals scavenging activities; Chicken lymphocytes
资金
- National Natural Science Foundation of China [30973838]
Response surface methodology along with Box-Behnken design was firstly applied to optimize the extraction conditions for the polysaccharides from the roots of Isatis tinctoria L. The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis. The 3-D response surface and the contour plots derived from the mathematical models were applied to determine the optimal conditions. The optimal conditions were extraction temperature (X-1) 99.5 degrees C, extraction time (X-2) 3.75 h and ratio of water to raw material (X-3) 11.84 (v/w). Under these conditions, the maximal observed value extraction yield of Isatis root polysaccharides (IRPSs) was (11.19 +/- 0.04)%, which was agreed with predicted value 11.17%. Pharmacological experiments indicated that IRPS have an appreciable ABTS radical scavenging ability in vitro and could increase IL-4 and IFN-gamma mRNA expression in chicken lymphocytes obtaining maximum promoted effects of 70% and 115%, respectively. This study may facilitate a deeper understanding of IRPS to provide theoretical references. (C) 2011 Elsevier Ltd. All rights reserved.
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