期刊
CARBOHYDRATE POLYMERS
卷 83, 期 2, 页码 688-696出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2010.08.040
关键词
Sea cucumber; Fucosylated chondroitin sulfate; Chondroitin sulfate; NMR; Anticoagulant activity
资金
- National Key Technology R&D Program of China [2008BAD94B05]
- National Natural Science Foundation of China [30871944]
- National High-Tech Research and Development Project of China [2007AA09180]
- Ministry of Science and Technology [2007DFA30980]
Fucosylated chondroitin sulfates (fCSs) were isolated from four sea cucumbers Pearsonothuria graeffet (Indo-Pacific) Stichopus tremulus (Western Indian Ocean) Holothuria vagabunda (Norwegian coast) and Isostichopus badionotus (Western Atlantic) The detailed sequences of fCSs particularly their fucose branches were characterized and compared H-1 and C-13 NMR of the polysaccharides clearly identified three different sulfation patterns on the branched fucoses 4-0-mono- 2 3-0-di- and 2 4-0-di-sulfation variously distributed in the four fCSs The chondroitin sulfate backbones were established based on the monosaccharide composition of the polysaccharides and two-dimensional NMR of their oligosaccharide fragments Anticoagulant activities of the four fCSs were assessed and compared The results indicated that the difference in their anticoagulant activities can be attributed to the difference in sulfation pattern of the fucose branch of the chondroitin sulfate and 2 4-0-disulfation is important for anticoagulant activity (C) 2010 Elsevier Ltd All rights reserved
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据