期刊
CARBOHYDRATE POLYMERS
卷 83, 期 3, 页码 1075-1081出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2010.08.071
关键词
Chitosan oligosaccharide; Spleen dendritic cell; TLR4; Immune response
资金
- NFSC [30970648]
To investigate the effects of chitosan oligosaccharide (COS) on dendritic cells (DCs) and the role of Toll-like receptor 4 (TLR4) in this immune process, Murine spleen CD11c(+) dendritic cells (SDCs) were isolated and cultured with S-COS and B-COS with polymeration degree of 3-7 and 7-16, respectively. The results showed that 8-COS up-regulated the expressions of MHCII and CD86 on SDCs, promoted the secretion of TNF-alpha from SDCs. SDCs treated with B-COS stimulated the proliferation of the CD4(+)T cells. However, these effects were not observed on SDCs treated with S-COS. Importantly, silencing the TLR4 expressions on SDCs by RNA interference approach attenuated the expression of CD86, MHCII on SDCs, and the secretion of TNF-alpha from SDCs, and the stimulating CD4(+)T cells proliferation capacity of SDCs induced by B-COS. These results suggest that B-COS, but not S-COS, promotes the activation of SDCs and TLR4 plays a bridge role in this process. (C) 2010 Elsevier Ltd. All rights reserved.
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