期刊
CARBOHYDRATE POLYMERS
卷 83, 期 2, 页码 813-817出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2010.08.058
关键词
Chitosan; Degree of deacetylation; Determination; Cellulase; Titration
资金
- National High Technology Research and Development Program of China (863 program) [2007AA091805]
- Qingdao University of Science and Technology
A novel method for determining the degree of deacetylation of chitosan is described This involves two-abrupt-change potentiometric titration preceded by enzymatic pretreatment (EP-TPT) with chitosanolytic enzyme (cellulase) to reduce the viscosity of chitosan solution Acidic titration is preferred for the rapid response within the two abrupt changes of pH Half-deacetylated chitosan (almost soluble in all pH range) was used to prove that the enzyme did not interfere with the measurement Compared with other methods such as H-1 NMR XRD FT-IR and linear potentiometric titration the proposed method is simple low-cost accurate reliable and easy to operate for industrial application (C) 2010 Elsevier Ltd All rights reserved
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