期刊
CARBOHYDRATE POLYMERS
卷 79, 期 1, 页码 192-199出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2009.07.049
关键词
Cellulose; Enzymatic hydrolysis; Media milling; Particle size
资金
- National Science Council of the Republic of China [NSC-93-2214-E-002-028]
The effect of particle size on enzymatic hydrolysis of cellulose has been investigated. The average size of microcrystalline cotton cellulose has been reduced to submicron scale by using a media mill. The milled products were further subjected to hydrolysis using cellulase. High cellulose concentration (7%) appeared to retard the size reduction and resulted in greater particles and smaller specific surface areas than those at low concentration (3%) with the same milling time. Initial rate method was employed to explore the rate of enzymatic hydrolysis of cellulose. The production rate of cellobiose was increased at least 5-folds due to the size reduction. The yield of glucose was also significantly increased depending upon the ratio of enzyme to substrate. A high glucose yield (60%) was obtained in 10-h hydrolysis when the average particle size was in submicron scale. (C) 2009 Elsevier Ltd. All rights reserved.
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