期刊
CARBOHYDRATE POLYMERS
卷 81, 期 1, 页码 49-56出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2010.01.054
关键词
Chitosan oligosaccharides; Vascular cell adhesion molecule-1; Intercellular adhesion molecule-1; Tumor necrosis factor-alpha; Mitogen-activated protein kinases; Human umbilical vein endothelial cells
资金
- National Programs for High Technology Research and Development [2006AA100313, 2007AA10Z343]
- Natural Science Fund of Chong Qing, China [KJ070304]
This study aimed to investigate the inhibitive effects of chitosan oligosaccharides (COS) on tumor necrosis factor (TNF)-alpha-induced over-expression of vascular adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) in human umbilical vein endothelial cells (HUVECs). We found that COS effectively inhibited TNF-alpha-induced expression of VCAM-1 and ICAM-1 at the level of transcription and translation. Signal transduction studies suggested that COS blocked TNF-alpha-induced activation of NF-kappa B, degradation of I kappa B alpha, and phosphorylation of p38 MAPK and ERK1/2. A further investigation showed that the NF-kappa B activation can be partly suppressed by p38 MAPK inhibitor (58203580) and ERK1/2 inhibitor (PD98059), which also ameliorated the mRNA expression of VCAM-1 and ICAM-1 in TNF-alpha-induced HUVECs. Additionally, COS decreased U937 monocyte adhesion to HUVECs induced by TNF-alpha. Our findings suggest that COS inhibit VCAM-1 and ICAM-1 production in activated HUVECs at least partly through the blockade of p38 and ERK1/2 signaling pathways. (C) 2010 Elsevier Ltd. All rights reserved.
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