期刊
CARBOHYDRATE POLYMERS
卷 82, 期 4, 页码 1161-1167出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2010.06.049
关键词
Sugar beet (Beta vulgaris) pectin; Endopolygalacturonase (endo-Pg); Rhamnogalacturonan (RG-I) region; Acid hydrolysis; Homogalacturonan (Hg) region
资金
- Region Pays de la Loire
- Biotechnology and Biological Sciences Research Council [BB/D01364X/1] Funding Source: researchfish
- BBSRC [BB/D01364X/1] Funding Source: UKRI
Acid extracted sugar beet (Beta vutgaris) pectin was subjected to enzymatic hydrolysis using fungal pectin methyl esterase (f-PME) and two endopolygalacturonanases (PGs land II). From the hydrolysate, the RG-I fraction was separated and purified by chromatographic techniques. This RG-I fraction was shown to be of high weight average molar mass (188,000 g/mol), but low intrinsic viscosity (36 ml/g), which is consistent with a random coil conformation(L-p = 1.4 nm). The HG fraction was prepared by mild acid hydrolysis of acid extracted pectin. The HG fraction was found to have a relatively low weight average molar mass (20,000 g/mol), but a rather high intrinsic viscosity (77 ml/g), which is consistent with the HG fraction being rigid in solution (L-p = 9.8 nm). Lower molar mass pectins are richer in HG regions and pectins of higher molar mass are richer in RG-I regions. We conclude that the degradation of the HG region has an important impact on intrinsic viscosity, but less on molar mass and the inverse is true for the degradation of RG-I region. This has important consequences in terms of the functionality of sugar beet pectin molecules. (C) 2010 Elsevier Ltd. All rights reserved.
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