Preparation and characterization of caffeic acid grafted chitosan/CPTMS hybrid scaffolds

In this study, preparation and characterization of caffeic acid grafted chitosan (CTS)/(3-chloropropyl)-trimethoxysilane (CPTMS) hybrid scaffolds were investigated. The grafting of caffeic acid onto CSC1.0 (CTS/CPTMS hybrid scaffold prepared in weight ratio of 1:1) not only increased its compressive strength but also enhanced its antibacterial, antioxidant and anti-cancer property. The caffeic acid grafted scaffolds exhibited higher anti Staphylococcus aureus and scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) property than CSC1.0 did. The compressive strength of the caffeic acid grafted scaffolds is 0.54 similar to 0.65 MPa, which was higher than that of CTS scaffold (0.14 MPa) and CSC1.0 (0.33 MPa). In culturing human osteosarcoma UMR-106 cells, the caffeic acid grafted scaffolds showed inhibitions on cell growth, alkaline phosphatase activity and cell attachment of UMR-106 cells. Caffeic acid grafting approach could be very promising for chitosan scaffolds to be used in hard-tissue engineering application. (C) 2009 Elsevier Ltd. All rights reserved.