期刊
CARBOHYDRATE POLYMERS
卷 77, 期 2, 页码 338-343出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2009.01.004
关键词
Exopolysaccharide (EPS); Geobacillus tepidamans; Microbial glucan; Polysaccharide thermostability; Anti-toxic activity
The thermophilic strain producing extracellular polysaccharide (EPS) was isolated from Velingrad hot spring, Bulgaria. StrainV264 showed 98% similarity in 16S rRNA gene sequence with Geobacillus tepidamans. Maltose was found to be the most appropriate carbon source for exopolysaccharide production. Maximum EPS production was reached in the early stationary phase. Highest EPS production of 111.4 mg L-1 was obtained in fermentor batch cultures at 300 rpm. The purified EPS expressed a high molecular weight and it was very stable at high temperature, showing a degradation at 280 degrees C. Chemical composition of the bio-polymer, determined by using a high pressure anion exchange-pulsed amperometric detector (HPAE-PAD), showed glucose as its major component. The polysaccharide was also investigated by spectroscopic methods (H-1 and C-13 NMR) which demonstrated the structure complexity of this biopolymer. (C) 2009 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据