期刊
CARBOHYDRATE POLYMERS
卷 77, 期 3, 页码 457-463出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2009.01.021
关键词
Bacterial cellulose; Gluconacetobacter xylinum; Multiwalled carbon nanotubes; Green fluorescent protein; Toxicity
资金
- BioGreen21 Program, Rural Development Administration, Republic of Korea [200810FTH010102001]
Carbon nanotubes are considered to be the ideal multi-functional filler, although there is some debate regarding their toxicity for bio-related applications. The bacteria, Cluconacetobacter xylinum, which produce bacterial cellulose, were cultured in a multiwalled carbon nanotube (MWCNT) dispersed Hestrin and Schramm (HS) medium by shaking incubation. The MWCNTs were functionalized with polyethylene glycol to prepare a stable MWCNT-dispersed HS medium and its stability was characterized by measuring the transmittance of a pulsed near infrared light. To investigate the toxicity of the MWCNTs to bacteria, we also introduced a green fluorescent protein gene into the bacteria and observed the fluorescence via confocal microscopy to confirm the presence of live bacteria in the MWCNT-dispersed HS medium. On the bases of the electron microscopy observations, a substantial number of MWCNTs were found to be well-dispersed and attached to the surface of the bacterial cellulose fibrils. (C) 2009 Elsevier Ltd. All rights reserved.
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