期刊
CARBOHYDRATE POLYMERS
卷 76, 期 1, 页码 17-22出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2008.09.016
关键词
Oleoyl-chitosan nanoparticles; Antibacterial dispersion system; Membrane integrity and permeability
资金
- NSFC [30770582]
- IST-CP [2006DFA33150]
- Ph.D. Programs Foundation of Ministry of Education of China [20070423013]
- National Research Foundation of Korea [과C6A2204] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
A novel chitosan antibacterial dispersion system was prepared by oleoyl-chitosan (O-chitosan) nanoparticles (OCNP) and the bactericidal activity against Escherichia coli and Staphylococcus aureus was evaluated by the enumeration of viable organisms at different incubation times. We further investigated the antimicrobial mode of OCNP using a combination of approaches, including cell integrity measurements, outer membrane (OM) and inner membrane (IM) permeabilization assays, SDS-PAGE and transmission electron microscopy (TEM). Results showed that when treated with OCNP, release of intracellular components quickly increased for both E. coli and S. aureus. OCNP also rapidly increased the 1-N-phenylnaphthylamine (NPN) uptake and the release of cytoplasmic beta-galactosidase via increasing the permeability of OM and IM. Besides, SDS-PAGE indicated the content of cellular soluble proteins decreased significantly in OCNP-treated bacteria. TEM observations demonstrated adsorption behaviors of OCNP on bacteria and extensive cell surface alterations of OCNP-treated bacteria. OCNP has potential value in the determination of antibacterial mechanism of chitosan. (C) 2008 Elsevier Ltd. All rights reserved.
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