期刊
CANCER SCIENCE
卷 104, 期 4, 页码 458-464出版社
WILEY-BLACKWELL
DOI: 10.1111/cas.12092
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资金
- Ministry of Science and Technology of China [2010CB912800, 2011CB504203]
- Natural Science Foundation of China [81230060, 81261140373, 81272893, 81172524]
- National S&T Major Special Project on New Drug Innovation of China [2011ZX09102-010-02]
- Science Foundation of Guangdong Province [S2012030006287]
- Guangdong Province Grants [2012B031800035, 2012J2200092]
- Sun Yat-sen University Training Project [11ykpy28, 11ykzd12]
Identification of new nasopharyngeal carcinoma (NPC) biomarkers is of great clinical value for the diagnosis and treatment of NPC. HOTAIR, a cancer-related long non-coding RNA, was tested and its prognostic value for NPC was evaluated. As determined using in situ hybridization (ISH), 91 of 160 (56.87%) paraffin-embedded NPC biopsies showed high expression levels of HOTAIR (staining index score 6). HOTAIR was upregulated in tumors with a large size (P=0.021), more advanced clinical stage (P=0.012) and increased lymph node tumor burden (P=0.005). Quantified using real-time PCR, HOTAIR expression levels in fresh tissue and paraffin-embedded samples were 5.2 similar to 48.4-fold higher compared with non-cancer tissue samples. Moreover, HOTAIR expression levels increased with clinical stage progression, which was consistent with ISH findings in the paraffin-embedded tissue. Most importantly, NPC patients with higher HOTAIR levels had a poor prognosis for overall survival using univariate and multivariate analysis. In addition, HOTAIR mediated the migration, invasion and proliferation of NPC cells in vitro. HOTAIR is a potential biomarker for the prognosis of NPC, and dysregulation of HOTAIR might play an important role in NPC progression. (Cancer Sci 2013; 104: 458-464)
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