Recognition of CG inversions in DNA triple helices by methylated 3H-pyrrolo[2,3-d] pyrimidin-2(7H)-one nucleoside analogues

Substituted 3H-pyrrolo[2,3-d] pyrimidin-2(7H)-one nucleoside analogues have been synthesised from 5-alkynyl-uridine derivatives, incorporated into triplex forming oligonucleotides (TFOs) and found to selectively bind CG inversions with enhanced affinity compared to T.