期刊
BIOLOGICAL PSYCHIATRY
卷 57, 期 1, 页码 102-106出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.biopsych.2004.09.026
关键词
endogenous; competition; synapse; positron emission tomography; primate
资金
- NATIONAL INSTITUTE OF MENTAL HEALTH [P50MH062185] Funding Source: NIH RePORTER
- NIMH NIH HHS [MH 62185] Funding Source: Medline
Background: The objective of this study was to evaluate the sensitivity of [C-11]-N,N-dimethyl-2-(2-amino-4-cyanophenylthio)benzylamine (DASB) binding to the brain serotonin transporter (SERT) to changes in endogenous serotonin (5-hydroxytryptamine [5-HT]) levels. A ligand sensitive to endogenous competition (EC) would enable the measurement of fluctuations of intrasynaptic 5-HT. A ligand insensitive to EC can provide a measure of SERT unaffected by levels of 5-HT. Alternatively, serotonin depletion could accelerate internalization of SERT and reduce binding. Methods. Eighteen (14 baseline and 9 tryptophan-depleted) positron emission tomography (PET) scans were carried out in two baboons (Papio anubis). A metabolite-corrected arterial input function was used to estimate the binding potential (BP = B-max/K-D). Results. Depletion of plasma tryptophan by a mean of 65% from the baseline (p =.03) reduces [C-11]-DASB BP in the six brain regions of interest (ROI). Lower DASB binding correlated with lower plasma tryptophan levels in the ROIs with higher SERT density. Conclusions. [C-11]-DASB binding to SERT in vivo rapidly declines in response to acute reduction in serotonin availability, contrary to what is predicted by a simple competition model. This rapid reduction in SERT availability may be due to accelerated transporter internalization.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据