期刊
BIOLOGY OF REPRODUCTION
卷 72, 期 1, 页码 119-126出版社
OXFORD UNIV PRESS INC
DOI: 10.1095/biolreprod.104.029520
关键词
estradiol receptor; gene regulation; gonadotropin-releasing hormone; neuroendocrinology; toxicology
An inhibition of vitellogenesis is observed in fish exposed to cadmium (Cd), either in natural or in experimental conditions. To investigate whether this correlates or not with modifications in the expression of several genes involved in reproduction, we have performed a study on juvenile rainbow trout (Oncorhynchus mykiss) exposed to waterborne Cd in combination with estradiol (E,). A relative reverse transcription-PCR protocol was used to evaluate the effect of Cd exposure on the expression of several genes. We quantified vitellogenin, rainbow trout estradiol receptor et (rtERalpha), short and long isoforms (rtERalphaS and rtERalphaL), mRNA levels in liver, and salmon GnRH1, salmon GnRH2, rtERalphaS, and rtERalphaL mRNA levels in the brain. in liver, Cd reduced the E-2-stimulated mRNA levels of vitellogenin as well as these of both rtERalpha isoforms in a dose-dependent manner. in brain tissue, our results indicate that rtERalpha mRNA levels are not enhanced by E-2. Cd treatments did not modify rtERalphaS isoform expression but reduced rtERaL expression in the brain. Focusing on the expression of salmon GnRH (sGnRH) genes, E-2 did not affect mRNA levels, but experiments with Cd alone greatly enhanced sGnRH 1 as well as sGnRH 2 gene expression in a dose-dependant manner. This study supports the idea that Cd is an important endocrine disrupter that could act through an inhibition of E-2-Stimlated genes in the liver and also through a central effect on sGnRH gene expression. Cd may affect a number of E-2 signaling pathways but could also affect the reproductive axis by nonestrogenic mechanisms.
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