期刊
FEMS MICROBIOLOGY LETTERS
卷 250, 期 1, 页码 9-17出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.femsle.2005.06.043
关键词
mycobacteria; cell division; green-fluorescent protein fusions; recombination; z-ring localization; fluorescence microscopy
类别
资金
- NIAID NIH HHS [AI48417, R01 AI048417, R01 AI048417-04, AI41406] Funding Source: Medline
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R01AI048417, R01AI041406] Funding Source: NIH RePORTER
We provide genetic evidence to show that the Mycobacterium tuberculosis FtsZ and FtsW proteins interact, and that these interactions are biologically relevant. Furthermore, we show by fluorescence microscopy that Mycobacterium smegmatis FtsW is part of its septasomal complex and colocalizes with FtsZ to the midcell sites. Colocalization experiments reveal that approximately 27% of the cells with septal Z-rings contain FtsW whereas 93% of the cells with FtsW bands are associated with FtsZ indicating that FtsW is late recruit to the septum, as in Eseherichia coli. Our results suggest that mycobacterial FtsZ can localize to the septum independent of FtsW, and that interactions of FtsW with FtsZ are critical for the formation of productive FtsZ-rings and the cell division process in mycobacteria. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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