期刊
JOURNAL OF CELL SCIENCE
卷 118, 期 1, 页码 233-242出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.01611
关键词
ataxin-1; spinocerebellar ataxia type 1; RNA processing; nuclear transport
类别
资金
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R37NS022920, R01NS022920] Funding Source: NIH RePORTER
- NINDS NIH HHS [NS22920] Funding Source: Medline
Spinocerebellar ataxia type 1 (SCAT) is a dominant neurodegenerative disease caused by the expression of mutant ataxin-1 containing an expanded polyglutamine tract. Ataxin-1 is a nuclear protein that localizes to punctate inclusions similar to neuronal nuclear inclusions seen in many polyglutamine expansion disease proteins. We demonstrate that ataxin-1 localization to inclusions and inclusion dynamics within the nucleus are RNA and transcription dependent, but not dependent on the polyglutamine tract. Ataxin-1 nuclear inclusions are distinct from other described nuclear bodies but recruit the mRNA export factor, TAP/NXF1, in a manner that is enhanced by cell heat shock. By FRAP protein dynamic studies in live cells, we found that wild-type, but not mutant, ataxin-1 was capable of nuclear export. These results suggest that the normal role of ataxin-1 may be in RNA processing, perhaps nuclear RNA export. Thus, nuclear retention of mutant ataxin-1 may be an important toxic gain of function in SCA1 disease.
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