期刊
BIOLOGICAL & PHARMACEUTICAL BULLETIN
卷 28, 期 9, 页码 1630-1634出版社
PHARMACEUTICAL SOC JAPAN
DOI: 10.1248/bpb.28.1630
关键词
lovastatin; C-reactive protein (CRP); nuclear factor-kappa B (NF-kappa B); CD40
The role of C-reactive protein (CRP) in atherogenesis has been supported by more recent data. Some studies have demonstrated marked up-regulation inflammatory responses in endothelial cells subjected to CRP. The nuclear factor-kappa B (NF-kappa B) signal transduction is known to play a key role in the expression of these proatherogenic entities. Statins have anti-inflammatory properties independent of their cholesterol-lowering effects. Therefore, we studied the effects of CRP and lovastatin on NF-kappa B activation in human umbilical vein endothelial cells (HUVECs). By using an electrophoretic mobility shift assays (EMSA), we found that CRP (50 mu g/ml) increased activation of NF-kappa B and degradation of inhibitory kappa B (I kappa B) in HUVECs, reaching a maximal effect after the incubation with CRP for 1 h. Lovastatin (10(-5) mol/l) diminished NF-kappa B activation induced by CRP. Furthermore, lovastatin may block NF-kappa B activation by causing a stabilization of the I kappa B-alpha in cellular cytoplasm with western blotting analysis. Preincubation of HUVECs with pyrrolidinethiocarbamate (PDTC, NF-kappa B inhibitor) diminished CD40 expression induced by CRP with flow cytometry. Our results suggest that CRP increases activation of NF-kappa B and induces CD40 expression in HUVECs partly via activation of NF-kappa B Lovastatin, through the inhibition of NF-kappa B activation, reduces the inflammation involved in the pathogenesis of atherosclerosis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据