期刊
PROTEOMICS
卷 5, 期 1, 页码 132-137出版社
WILEY
DOI: 10.1002/pmic.200400919
关键词
biotinylation; insulin receptor substrate-1; tandem affinity purification tag technique
资金
- NCI NIH HHS [CA86940] Funding Source: Medline
- NIA NIH HHS [AG20956] Funding Source: Medline
- NATIONAL INSTITUTE ON AGING [R01AG020956] Funding Source: NIH RePORTER
The tandem affinity purification (TAP) tag technique has been used with success to identify under nondenaturing conditions protein complexes in yeast. The technique can be used in mammalian cells, but we found that the original technique does not yield enough recovery for the identification of proteins when mammalian cells growing in monolayer have to be used. We present here a modified TAP tag technique that allows sufficient recovery of proteins from mouse fibroblasts growing in monolayer cultures. The recovery allows protein identification by mass spectrometry.
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