期刊
HYDROBIOLOGIA
卷 532, 期 -, 页码 103-110出版社
SPRINGER
DOI: 10.1007/s10750-004-9450-3
关键词
enzyme; peatlands; MUF; chitin; methodology
A fluorogenic model substrate, methylumbelliferyl-N-acetylglucosamine was used to measure N-acetylglucosaminidase activities in wetland soils. The enzyme activity exhibited substrate saturation around 200 muM, and an approximate linearity for at least 90 min incubation time. The effects of temperature, pH, toluene, and gamma-radiation on the enzyme were also determined. The method was applied to 32 different wetland samples from both freshwater wetlands and salt-water wetlands. Overall, the activities were the lowest in bogs and much higher in freshwater marshes and flooded grasslands. The variations of the activity were not explained by a single environmental variable. However, among peatland samples (e.g., bogs and fens) only, specific enzyme activity per organic matter exhibited a significant correlation with N-acetylglucosaminidase activity, suggesting the importance of the enzyme activity in organic matter dynamics in such systems.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据