期刊
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
卷 115, 期 1, 页码 103-109出版社
MOSBY-ELSEVIER
DOI: 10.1016/j.jaci.2004.10.007
关键词
airway responsiveness; bronchoalveolar lavage; cytokines; IgE; leptin; ovalbumin
资金
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [P01HL033009, T32HL007118] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES [P30ES000002] Funding Source: NIH RePORTER
- NHLBI NIH HHS [HL07118, HL33009] Funding Source: Medline
- NIEHS NIH HHS [ES00002] Funding Source: Medline
Background: Epidemiologic data indicate that the incidence of asthma is increased in obese patients. Objective: Because the serum levels of the satiety hormone and proinflammatory cytokine leptin are increased in obese individuals, we sought to determine whether leptin can augment allergic airway responses. Methods: We sensitized and challenged BALB/cJ mice with ovalbumin. Alzet(R) micro-osmotic pumps were implanted in the mice to deliver a continuous infusion of either saline or leptin (1.75 mug/g/d). Two days later, the mice were challenged with either aerosolized saline or ovalbumin once per day for 3 days. We measured airway responsiveness, performed bronchoalveolar ravage, and obtained blood to measure serum leptin and IgE 24 or 48 hours after the last challenge. Results: Leptin infusion increased serum leptin concentrations, which were increased further after ovalbumin sensitization and challenge. Ovalbumin challenge increased bronchoalveolar lavage fluid cells and cytokines, serum IgE, lung cytokine mRNA expression, and responses to inhaled, aerosolized methacholine. It is important to note that the changes in methacholine responsiveness and IgE were augmented in leptin- versus saline-infused mice. Conclusions: These results indicate that serum leptin is increased during allergic reactions in the airways and may play a role in the relationship between obesity and asthma.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据