期刊
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
卷 19, 期 24, 页码 3701-3704出版社
WILEY
DOI: 10.1002/rcm.2243
关键词
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A new method of electrospray-assisted laser desorption/ionization (ELDI) mass spectrometry, which combines laser desorption with post-ionization by electrospray, was applied to rapid analysis of solid materials under ambient conditions. Analytes were desorbed from solid metallic and insulating substrata using a pulsed nitrogen laser. Post-ionization produced high-quality mass spectra characteristic of electrospray, including protein multiple charging. For the first time, mass spectra of intact proteins were obtained using laser desorption without adding a matrix. Bovine cytochrome c and an illicit drug containing methaqualone were chosen in this study to demonstrate the applicability of ELDI to the analysis of proteins and synthetic organic compounds. Copyright (c) 2005 John Wiley & Sons, Ltd.
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