期刊
CELL CYCLE
卷 4, 期 1, 页码 155-165出版社
TAYLOR & FRANCIS INC
DOI: 10.4161/cc.4.1.1347
关键词
cyclin-dependent kinase; RINGO; Speedy; Cdc2; Cdk2; cell cycle; cyclin
类别
资金
- NIGMS NIH HHS [GM47830] Funding Source: Medline
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM047830] Funding Source: NIH RePORTER
In addition to their activation via binding to cyclins, cyclin-dependent kinases (CDKs) can be activated via binding to a novel cell cycle regulator termed Speedy/Ringo, which shows no apparent similarity to cyclins. The first Speedy/Ringo protein was found to be essential for Xenopus oocyte maturation and a human homolog (Spy1, herein called Speedy/Ringo A1) regulates S-phase entry and cell survival after DNA damage in cultured somatic cells. We have identified a Speedy/Ringo-like gene in the most primitive branching clade of chordates ( Ciona intestinalis), as well as four mammalian homologs. Of the mammalian proteins, two, Speedy/Ringo A and C, bind to Cdc2 and Cdk2, whereas Speedy/Ringo B binds preferentially to Cdc2. Despite their distinct CDK-binding preferences, both Speedy/Ringo A and B can promote the maturation of Xenopus oocytes and all three Speedy/Ringo proteins can bind to and activate CDKs in vivo. These mammalian Speedy/ Ringo proteins exhibit distinct tissue expression patterns, though all three are enriched in testis, consistent with the initial observation that Xenopus Speedy/ Ringo functions during meiosis. Speedy/ Ringo A is widely expressed in tissues and cell lines. Speedy/ Ringo B expression appears to be testis-specific. Speedy/ Ringo C is expressed in diverse tissues, particularly those that undergo polyploidization. All Speedy/ Ringo proteins share a highly conserved similar to 140-aa domain we term the Speedy/ Ringo box that is essential for CDK binding. Point mutations in this domain abolish CDK binding. Besides the central Speedy/ Ringo box, Speedy/ Ringo A contains a C-terminal portion, which promotes CDK activation, and an N-terminal portion, which is dispersible for both CDK binding and activation but that influences protein expression. The existence of this growing family of CDK activators suggests that Speedy/ Ringo proteins may play as complex a role in cell cycle control as the diverse family of cyclins.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据