期刊
OSTEOPOROSIS INTERNATIONAL
卷 16, 期 1, 页码 101-108出版社
SPRINGER LONDON LTD
DOI: 10.1007/s00198-004-1649-7
关键词
adipogenesis; dexamethasone; mesenchymal cells; osteogenesis; osteonecrosis; vascular endothelial growth factor
We have studied the mechanism of steroid-induced osteonecrosis by examining the effect of dexamethasone on a multipotential cell line, D1, which is derived from bone marrow and is capable of differentiating into either the osteoblast or the adipocyte lineage. The expression of bone cell and fat cell transcription factors Cbfal/Runx2 and PPARgamma2,. were determined. Osteocalcin promoter activity was measured by co-transfecting the cells with the phOC-1uc and pSV beta-Gal plasmids. Dexamethasone increased PPARgamma2 gene expression 2-fold. while Cbfal/Runx2 gene expression and osteocalcin promoter activity decreased by 50-60%, and VEGF protein, measured by ELISA, decreased by 55%. These changes indicate enhanced adipogenesis and decreased osteogenesis by mesenchymal cells in vitro. together with a decrease in VEGF, a potent angiogeneic factor, suggesting that dexamethasone may shunt uncommitted osteoprogenitor cells in marrow from osteoblastic differentiation into the adipocytic pathway, leading to diminished vascularization and eventual osteonecrosis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据