期刊
JOURNAL OF SEPARATION SCIENCE
卷 28, 期 1, 页码 39-44出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200401932
关键词
titania-coated silica; monolithic silica; phosphorus-containing compounds; high performance; liquid chromatography
A method of preparing titania-coated monolithic silica stationary phase has been developed to achieve liquid chromatographic separation of phosphorus-containing compounds, which have recently been attracting increasing attention in biochemical research. The titania-coated silica columns exhibited efficient separation with low pressure drop, which is a typical feature of monolithic structures, and also possessed phospho-selectivity, which is a unique property of the titania surface. The material characteristics of titania-coated monolithic silica were examined, and then resin-clad columns were applied to the HPLC analysis of phosphorylated compounds. Highly efficient separation of phosphorylated substances indicated that the novel titania-coated monolithic silica column will find applications as a useful tool in the field of biochemistry, especially in post-genomic analyses.
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