期刊
LASERS IN SURGERY AND MEDICINE
卷 36, 期 1, 页码 2-7出版社
WILEY
DOI: 10.1002/lsm.20130
关键词
apoptosis; caspase-3; fluorescence resonance energy transfer (FRET); high fluence low-power laser; irradiation (high fluence LPLI); living cells
Background and Objectives: Low-power laser irradiation (LPLI) has been used for therapies such as curing spinal cord injury, healing wound etc. Yet, the mechanism of LPLI remains unclear. In order to determine the effects of high fluence LPLI on cell growth and caspase-3 activity, we have measured the dynamics of caspase-3 activity during cell apoptosis induced by high fluence LPLI treatment. Study Design/Materials and Methods: He-Ne laser was used to irradiate human lung adenocarcinoma cells (ASTC-a-1). Cell Counting Kit-8 was used for cytotoxicity assay. A fluorescent microscope was used to perform fluorescence resonance energy transfer (FRET) imaging. A luminescence spectrometer was used to acquire the fluorescent emission spectrum. Statistical analysis was performed with Student's paired t-test. Results: Cytotoxicity assay showed that when light irradiation fluence exceeded 60 J/cm(2), LPLI treatment induced ASTC-a-1 cell apoptosis in a fluence-dependent manner. FRET imaging and spectrofluorometric analysis demonstrated that caspase-3 was activated during high fluence LPLI-induced cell apoptosis. Conclusions: Using FRET technique, we have reported that high fluence LPLI can induce human lung adenocarcinoma cells (ASTC-a-1) apoptosis. The activation of caspase-3 plays an important role in the apoptotic process. (C) 2005Wiley-Liss, Inc.
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