期刊
MOLECULAR ECOLOGY NOTES
卷 5, 期 3, 页码 611-612出版社
WILEY
DOI: 10.1111/j.1471-8286.2005.01009.x
关键词
nematodes; 185 ribosomal RNA (SSU); specific amplification; DNA barcoding
A set of polymerase chain reaction primers were designed, which amplify a c.1 kb fragment of the 18S ribosomal DNA gene, and are specific to the phylum Nematoda. These have proven useful in isolating nematode genes from samples mixed with other biological material, particularly with application to DNA barcoding. Optimal reaction conditions are described. These primers have successfully amplified the correct fragment from a wide phylogenetic range of nematodes, and have so far generated no sequences from any other organismal group.
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