期刊
JOURNAL OF PROTEOME RESEARCH
卷 4, 期 5, 页码 1867-1871出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr050084g
关键词
expression analysis; proteomics; differential gel electrophoresis; replication; nested ANOVA
In expression proteomics, the samples utilized within an experimental design may include technical, biological, or pooled replicates. This manuscript discusses various experimental designs and the conclusions that can be drawn from them. Specifically, it addresses the impact of mixing replicate types on the statistical analysis which can be performed. This study focuses on difference gel electrophoresis (DiGE), but the issues are equally applicable to all quantitative methodologies assessing relative changes in protein expression.
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